酮基移换酶样蛋白1抗体-产品资讯-资讯-生物在线

酮基移换酶样蛋白1抗体

作者:上海雅吉生物科技有限公司 2020-11-05T09:10 (访问量:1922)

中文名称 酮基移换酶样蛋白1抗体
别 名 TK 2; TK2; TKR; TKT 2; TKT2; TKTL1; TKTL1_HUMAN; Transketolase 2; Transketolase like 1; Transketolase like protein 1; Transketolase related protein; Transketolase-like protein 1; Transketolase-related protein; Transketolase2.
研究领域 肿瘤 信号转导 肿瘤细胞生物标志物
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 65 kDa
细胞定位 细胞核 细胞浆
性 状 Liquid
浓 度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human TKTL1:101-200/596
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The protein encoded by this gene is a transketolase that acts as a homodimer and catalyzes the conversion of sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate to D-ribose 5-phosphate and D-xylulose 5-phosphate. This reaction links the pentose phosphate pathway with the glycolytic pathway. Variations in this gene may be the cause of Wernicke-Korsakoff syndrome. Three transcript variants encoding different isoforms have been found for this gene.

Function:
Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate

Subunit:
Homodimer

Subcellular Location:
Cytoplasm. Nucleus. Note=Predominantly cytoplasmic and to a lesser extent also nuclear.

Tissue Specificity:
Expressed in fetal and adult heart, brain, lung, liver, and kidney, and in adult placenta, skeletal muscle and pancreas. Up-regulated in various epithelial tumors.

Similarity:
Belongs to the transketolase family.

SWISS:
P51854

Gene ID:
8277

Database links:

Entrez Gene: 8277 Human

Omim: 300044 Human

SwissProt: P51854 Human

Unigene: 102866 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片 Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-TKTL1 (bs-6284R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
Sample:
293T(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
Primary: Anti-TKTL1 (bs-6284R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TKTL1) Polyclonal Antibody, Unconjugated (bs-6284R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TKTL1) Polyclonal Antibody, Unconjugated (bs-6284R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

上海雅吉生物科技有限公司 商家主页

地 址: 上海市闵行区元江路5500号第1幢5658室

联系人: 王源

电 话: 15301693058

传 真: 021-34661275

Email:yajikit@163.com

相关咨询

人原代附睾管上皮细胞 (2021-07-08T14:16 浏览数:9688)

雅吉生物热烈庆祝中国建党100周年 (2021-07-02T09:17 浏览数:5297)

15P-1 (小鼠睾丸上皮细胞) (STR鉴定正确) (2021-06-23T09:59 浏览数:7799)

客户细胞培养过程中常见的问题说明 (2021-06-18T09:54 浏览数:9933)

免疫荧光鉴定步骤 (2021-06-18T09:54 浏览数:11429)

人真皮成纤维细胞的分离培养制备方式 (2021-06-17T09:04 浏览数:7980)

原代角质形成细胞的分离培养方法 (2021-06-17T09:03 浏览数:11579)

钻石探头量子显微镜可以探索纳米微观世界的奥秘 (2021-06-16T09:24 浏览数:8583)

过氧化物酶体活化受体可以加剧肿瘤形成 (2021-06-16T09:23 浏览数:8563)

式细胞术数据分析之——圈门技巧 (2021-06-15T10:11 浏览数:8933)

ADVERTISEMENT