朊病毒蛋白(PRNP)重组蛋白

YB680Mu011
Recombinant Prion Protein (PRNP)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 朊病毒蛋白(PRNP)重组蛋白[ PROPERTIES ]
Residues: Lys23~Ser230 (Accession # P04925),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Cell membrane;
Lipid-anchor, GPI-anchor. Golgi apparatus.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
 朊病毒蛋白(PRNP)重组蛋白pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 9.6
Predicted Molecular Mass: 26.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 朊病毒蛋白(PRNP)重组蛋白[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KKRPKPGG WNTGGSRYPG
QGSPGGNRYP PQGGTWGQPH GGGWGQPHGG SWGQPHGGSW GQPHGGGWGQ
GGG TH NQW NK PSKPKT N LK H VAGAAAAGAV VG G LGGYM LG SAMS RPMI HF
GNDWEDRYYR ENMYRYPNQV YYRPVDQYSN QNNFVHDCVN ITIKQHTVTT TTKGENFTET
DVKMMERVVE QMCVTQYQKE SQAYYDGRRS