基质金属蛋白酶20(MMP20)重组蛋白

YB855Mu012
Recombinant Matrix Metalloproteinase 20 (MMP20)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
基质金属蛋白酶20(MMP20)重组蛋白[ PROPERTIES ]
Residues: Val137~Tyr398
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P57748
Host: E. coli
Subcellular Location: Secreted, extracellular
space, extracellular matrix.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
基质金属蛋白酶20(MMP20)重组蛋白pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 7.0
Predicted Molecular Mass: 33.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
基质金属蛋白酶20(MMP20)重组蛋白precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
VDKA IQMALHAWST AVPLNFVRIN SGEADIMISF ETGDHGDSYP FDGPRGTLAH
AFAPGEGLGG DTHFDNAEKW TMGTNGFNLF TVAAHEFGHA LGLGHSTDPS ALMYPTYKYQ
NPYRFHLPKD DVKGIQALYG PRKIFPGKPT MPHIPPHKPS IPDLCDSSSS FDAVTMLGKE
LLFFKDRIFW RRQVHLPTGI RPSTITSSFP QLMSNVDAAY EVAERGIAFF FKGPHYWVTR
GFHMQGPPRT IYDFGFPRHV QRIDAAVY