RAD9A (phospho S277) polyclonal antibody
产品名称： RAD9A (phospho S277) polyclonal antibody
英文名称： RAD9A (phospho S277) polyclonal antibody
产品编号： PAB0582
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of RAD9A.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S277 of human RAD9A.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Accumulation of hRad9 protein in the nuclei of nonsmall cell lung carcinoma cells.
Maniwa Y, Yoshimura M, Bermudez VP, Yuki T, Okada K, Kanomata N, Ohbayashi C, Hayashi Y, Hurwitz J, Okita Y.Cancer. 2005 Jan 1;103(1):126-32.
- 2.
- The human Rad9-Rad1-Hus1 checkpoint complex stimulates flap endonuclease 1.
Wang W, Brandt P, Rossi ML, Lindsey-Boltz L, Podust V, Fanning E, Sancar A, Bambara RA.Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16762-7. Epub 2004 Nov 19.
- 3.
- The human Rad9 checkpoint protein stimulates the carbamoyl phosphate synthetase activity of the multifunctional protein CAD.
Lindsey-Boltz LA, Wauson EM, Graves LM, Sancar A.Nucleic Acids Res. 2004 Aug 23;32(15):4524-30. Print 2004.
- Applications
- Western Blot (Cell lysate)
- The RAD9A (phospho S277) polyclonal antibody (Cat # PAB0582) is used in Western blot to detect Phospho-RAD9A-S277 in Y-79 cell lysate
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with RAD9A (phospho S277) polyclonal antibody (Cat # PAB0582) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 5883
- Protein Accession#:
- NP_004575;Q99638
- Gene Name:
- RAD9A
- Gene Alias:
- RAD9
- Gene Description:
- RAD9 homolog A (S. pombe)
- Omim ID:
- 603761
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq
- Other Designations:
- RAD9 homolog,cell cycle checkpoint control protein