Shh (Mouse) Recombinant Protein
产品名称： Shh (Mouse) Recombinant Protein
英文名称： Shh (Mouse) Recombinant Protein
产品编号： P4621
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- 所在区域 : 台湾
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- Specification
- Product Description:
- Mouse Shh (Q15465) recombinant protein expressed in Escherichia coli.
- Sequence:
- MIIGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG
- Theoretical MW (kDa):
- 19.8
- Form:
- Lyophilized
- Preparation Method:
- Escherichia coli expression system
- Endotoxin Level:
- < 0.01 ng/ug or < 0.1 EU/ug.
- Activity:
- The activity is determined by the dose-dependent increase of alkaline phosphatase activity in C3H/10T1/2 fibroblasts (CCL-226). The expected ED50 for this effect is 0.48-0.72 ug/mL.
- Storage Buffer:
- Lyophilized with 10 mM Na2PO4, pH 7.5.
- Storage Instruction:
- Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Note:
- Result of activity analysis
-
Serial dilutions of mouse Shh, starting at 5 ug/mL, were added to with CCL-226 cells in the presence of 1 uM Retinoic Acid. Alkaline phosphatase was measured and the linear portion of the curve was us used to calculate the ED50.
- Application Image
- Functional Study
- SDS-PAGE